{"id":533,"date":"2023-05-30T13:52:08","date_gmt":"2023-05-30T05:52:08","guid":{"rendered":"http:\/\/ly.com.cn\/?p=533"},"modified":"2023-05-30T13:52:08","modified_gmt":"2023-05-30T05:52:08","slug":"%e7%90%bc%e8%84%82%e7%b3%96%e5%87%9d%e8%83%b6%e7%94%b5%e6%b3%b3","status":"publish","type":"post","link":"http:\/\/ly.com.cn\/?p=533","title":{"rendered":"\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3"},"content":{"rendered":"<section class=\"wpb-content-wrapper\"><p>[vc_row][vc_column][vc_column_text]\u5bf9\u4e8e\u5206\u5b50\u91cf\u5927\u7684\u6837\u54c1\uff0c\u5982\u5927\u5206\u5b50\u6838\u9178\u3001\u75c5\u6bd2\u7b49\uff0c\u4e00\u822c\u91c7\u7528\u5b54\u5f84\u8f83\u5927\u7684\u743c\u8102\u7cd6\u51dd\u80f6\u8fdb\u884c\u7535\u6cf3\u5206\u79bb\u3002<\/p>\n<p>\u743c\u8102\u7cd6\u662f\u7531\u534a\u4e73\u7cd6\u53ca\u5176\u884d\u751f\u7269\u6784\u6210\u7684\u4e2d\u6027\u7269\u8d28\uff0c\u4e0d\u5e26\u7535\u8377\u3002\u743c\u8102\u7cd6\u662f\u76f4\u94fe\u591a\u7cd6\uff0c\u5b83\u7531D-\u534a\u4e73\u7cd6\u548c3,6-\u8131\u6c34-L-\u534a\u4e73\u7cd6\u7684\u6b8b\u57fa\u4ea4\u66ff\u6392\u5217\u7ec4\u6210<\/p>\n<p>\u743c\u8102\u7cd6\u4e0e\u743c\u8102\u7684\u533a\u522b<\/p>\n<p>\u743c\u8102\u662f\u7531\u743c\u8102\u7cd6\uff08agarose\uff09\u548c\u743c\u8102\u679c\u80f6\uff08agaropectin\uff09\u7ec4\u6210\u7684\u3002\u743c\u8102\u7cd6\u7684\u7ed3\u6784\u5355\u5143\u662f D-\u534a\u4e73\u7cd6\u548c3.6-\u8131\u6c34-L-\u534a\u4e73\u7cd6\uff1b\u743c\u8102\u679c\u80f6\u662f\u7531\u8bb8\u591a\u66f4\u5c0f\u7684\u5206\u5b50\u7ec4\u6210\u7684\u5f02\u8d28\u6df7\u5408\u7269\u3002\u5b83\u4eec\u7684\u7ed3\u6784\u76f8\u4f3c\uff0c\u4f46\u743c\u8102\u679c\u80f6\u5e26\u786b\u9178\u6839\u548c\u7fa7\u57fa\u7ec4\u5206\uff0c\u51dd\u80f6\u80fd\u529b\u5dee\u3002 \u5728\u743c\u8102\u7cd6\u5236\u5907\u8fc7\u7a0b\u4e2d\u9700\u8981\u628a\u743c\u8102\u679c\u80f6\u5c3d\u91cf\u53bb\u9664\uff0c\u5426\u5219\u743c\u8102\u7cd6\u6709\u53ef\u80fd\u5b58\u5728\u6781\u5fae\u91cf\u786b\u9178\u6839\u548c\u4e19\u916e\u9178\u53d6\u4ee3\u7535\u79bb\u57fa\u56e2\uff0c\u5c31\u4f1a\u9020\u6210\u7535\u5185\u6e17\uff0c\u7535\u5185\u6e17\u5bf9\u8d28\u70b9\u7684\u79fb\u52a8\u4ea7\u751f\u5f71\u54cd\u3002\u8d28\u91cf\u8f83\u597d\u7684\u743c\u8102\u7cd6\u786b\u9178\u6839\u542b\u91cf\u6bd4\u8f83\u4f4e\uff0c\u901a\u5e38\u57280.2%\u4ee5\u4e0b\uff0c\u7535\u5185\u6e17\u6bd4\u8f83\u5c0f\uff0c\u901a\u5e38\u57280.13%\u4ee5\u4e0b\u3002 \u7b80\u8a00\u4e4b\uff0c\u743c\u8102\u7cd6\u662f\u4ece\u743c\u8102\u4e2d\u7cbe\u7ec6\u63d0\u53d6\u7684\uff0c\u6709\u81ea\u8eab\u72ec\u7279\u7684\u6027\u8d28\uff0c\u6240\u4ee5\u743c\u8102\u7cd6\u8981\u6bd4\u743c\u8102\u8d35\u5f97\u591a\u3002<\/p>\n<p>\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\uff1a\u5b9e\u9a8c\u539f\u7406<\/p>\n<p>1.DNA\u5206\u5b50\u5728\u78b1\u6027\u7f13\u51b2\u6db2\u4e2d\u5e26\u8d1f\u7535\u8377\uff0c\u5728\u5916\u52a0\u7535\u573a\u4f5c\u7528\u4e0b\u5411\u6b63\u6781\u6cf3\u52a8\u3002<\/p>\n<p>2.DNA\u5206\u5b50\u5728\u743c\u8102\u7cd6\u51dd\u80f6\u4e2d\u6cf3\u52a8\u65f6\uff0c\u6709\u7535\u8377\u6548\u5e94\u4e0e\u5206\u5b50\u7b5b\u6548\u5e94\u3002\u4e0d\u540cDNA\u7684\u5206\u5b50\u91cf\u5927\u5c0f\u53ca\u6784\u578b\u4e0d\u540c\uff0c\u7535\u6cf3\u65f6\u7684\u6cf3\u52a8\u7387\u5c31\u4e0d\u540c\uff0c\u4ece\u800c\u5206\u51fa\u4e0d\u540c\u7684\u533a\u5e26\u3002<\/p>\n<p>3.DNA \u5206\u5b50\u7684\u8fc1\u79fb\u901f\u5ea6\u4e0e\u76f8\u5bf9\u5206\u5b50\u8d28\u91cf\u7684\u5bf9\u6570\u503c\u6210\u53cd\u6bd4\u5173\u7cfb\u3002\u51dd\u80f6\u7535\u6cf3\u4e0d\u4ec5\u53ef\u5206\u79bb\u4e0d\u540c\u76f8\u5bf9\u5206\u5b50\u8d28\u91cf\u7684DNA \uff0c\u4e5f\u53ef\u4ee5\u5206\u79bb\u76f8\u5bf9\u5206\u5b50\u8d28\u91cf\u76f8\u540c\uff0c\u4f46\u6784\u578b\u4e0d\u540c\u7684DNA \u5206\u5b50\u3002<\/p>\n<p>\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3<\/p>\n<p>1. \u6839\u636e\u7535\u6cf3\u9700\u8981\uff0c\u914d\u7f6e\u5408\u9002\u6d53\u5ea6\u7684\u7535\u6cf3\u53ca\u5236\u80f6\u7f13\u51b2\u6db2\u3002\u4e00\u822c\u4e3a 1\u00d7 TAE\u62160.5\u00d7TBE\u3002 \u6ce8\u610f\uff1a\u7528\u4e8e\u7535\u6cf3\u7684\u7f13\u51b2\u6db2\u548c\u7528\u4e8e\u5236\u80f6\u7684\u7f13\u51b2\u6db2\u5fc5\u987b\u662f\u76f8\u540c\u7684\u3002<\/p>\n<p>2. \u6839\u636e\u5236\u80f6\u91cf\u53ca\u51dd\u80f6\u6d53\u5ea6\uff0c\u5728\u52a0\u6709\u4e00\u5b9a\u91cf\u7684\u7535\u6cf3\u7f13\u51b2\u6db2\u7684\u4e09\u89d2\u9525\u74f6\u4e2d\uff0c\u52a0\u5165\u51c6\u786e\u79f0\u91cf\u7684\u743c\u8102\u7cd6\u7c89\u3002 \u6ce8\u610f\uff1a\u603b\u6db2\u4f53\u91cf\u4e0d\u5b9c\u8d85\u8fc7\u4e09\u89d2\u9525\u74f6\u768450%\u5bb9\u91cf\u3002<\/p>\n<p>3. \u5728\u5fae\u6ce2\u7089\u4e2d\u52a0\u70ed\u6eb6\u89e3\u743c\u8102\u7cd6\u5e94\u6ce8\u610f\uff1a\u743c\u8102\u7cd6\u5145\u5206\u5b8c\u5168\u6eb6\u89e3\uff0c\u5426\u5219\uff0c\u4f1a\u9020\u6210\u7535\u6cf3\u56fe\u50cf\u6a21\u7cca\u4e0d\u6e05\u3002\u52a0\u70ed\u65f6\u5982\u80f6\u6db2\u5267\u70c8\u6cb8\u817e\u53d1\u6ce1\uff0c\u505c\u6b62\u52a0\u70ed\u3002\u5fae\u6ce2\u7089\u4e2d\u52a0\u70ed\u65f6\u95f4\u4e0d\u5b9c\u8fc7\u957f\u3002<\/p>\n<p>4.\u4f7f\u6eb6\u6db2\u51b7\u5374\u81f360\u2103\u5de6\u53f3\uff0c\u5982\u9700\u8981\u53ef\u5728\u6b64\u65f6\u52a0\u5165\u6eb4\u5316\u4e59\u952d(EB)\u6eb6\u6db2\u4f7f\u5176\u7ec8\u6d53\u5ea6\u4e3a0.5ug\/ml\uff0c\u5e76\u5145\u5206\u6df7\u5300\u2014\u2014\u4e0d\u63d0\u5021\u4f7f\u7528\u3002 \u6ce8\u610f\uff1a\u6eb4\u5316\u4e59\u952d\u662f\u4e00\u79cd\u81f4\u764c\u7269\u8d28\u3002\u4f7f\u7528\u542b\u6709\u6eb4\u5316\u4e59\u952d\u7684\u6eb6\u6db2\u65f6\uff0c\u8bf7\u6234\u7528\u624b\u5957\u3002\u6eb4\u5316\u4e59\u952d\u5728\u53ef\u89c1\u5149\u4e0b\u4f1a\u5206\u89e3\u3002<\/p>\n<p>5.\u5c06\u743c\u8102\u7cd6\u6eb6\u6db2\u5012\u5165\u5236\u80f6\u6a21\u4e2d\uff0c\u7136\u540e\u5728\u9002\u5f53\u4f4d\u7f6e\u5904\u63d2\u4e0a\u68b3\u5b50\u3002\u51dd\u80f6\u539a\u5ea6\u4e00\u822c\u57285\uff0d8 mm \u4e4b\u95f4\u3002 \u6ce8\u610f\uff1a\u4e0d\u8981\u4ea7\u751f\u6c14\u6ce1\uff0c\u6eb6\u6db2\u6e29\u5ea6\u592a\u9ad8(&gt;60\u2103)\uff0c\u4f1a\u4f7f\u5f97\u6c34\u5206 \u8fc7\u5206\u84b8\u53d1\uff0c\u6539\u53d8\u51dd\u80f6\u79bb\u5b50\u6d53\u5ea6\uff0c\u5f71\u54cd\u7535\u6cf3\u6548\u679c\u3002<\/p>\n<p>6.\u5728\u5ba4\u6e29\u4e0b\u4f7f\u80f6\u51dd\u56fa\uff08\u5927\u7ea630 \u5206\u949f\uff09\uff0c\u7136\u540e\u653e\u7f6e\u4e8e\u7535\u6cf3\u69fd\u4e2d\u8fdb\u884c\u7535\u6cf3\u3002 \u6ce8\u610f\uff1a\u51dd\u80f6\u4e0d\u7acb\u5373\u4f7f\u7528\u65f6\uff0c\u7528\u4fdd\u9c9c\u819c\u5c06\u51dd\u80f6\u5305\u597d\u57284\u2103\u4e0b\u4fdd\u5b58\uff0c\u6216\u8005\u653e\u5728\u5236\u80f6\u7684\u7f13\u51b2\u6db2\u4e2d\uff0c\u4e00\u822c\u53ef\u4fdd\u5b582\uff5e5 \u5929\u3002<\/p>\n<p>\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3<\/p>\n<p>1 \u4eea \u5668\uff1a\u6c34\u5e73\u7535\u6cf3\u4eea\u3001\u4e2d\u4f4e\u538b\u7535\u6cf3\u4eea\u7535\u6e90\u3001\u51dd\u80f6\u6210\u50cf \u5206\u6790\u7cfb\u7edf 2 \u914d\u5957\u8bd5\u5242\uff1a<\/p>\n<p>\uff081\uff09\u4ecb \u8d28\uff1a\u743c\u8102\u7cd6\u51dd\u80f6<\/p>\n<p>\uff082\uff09\u7f13\u51b2\u6db2\uff1aTAE\u7f13\u51b2\u6eb6\u6db2\uff08Tris\u78b1\u3001\u4e59\u4e8c\u80fa\u56db\u4e59\u9178 \u4e8c\u94a0\u4e8c\u6c34\u3001\u51b0\u4e59\u9178\uff09 TBE\u7f13\u51b2\u6eb6\u6db2\uff08 Tris\u78b1\u3001\u4e59\u4e8c\u80fa\u56db\u4e59\u9178 \u4e8c\u94a0\u4e8c\u6c34\u3001\u787c\u9178\uff09<\/p>\n<p>\uff083\uff09\u4e0a\u6837\u7f13\u51b2\u6db2\uff1a 6\u00d7DNA Loading Buffer\uff08DNA\u6837 \u54c1\u4e13\u7528\u5305\u542b\uff1aEDTA\u3001\u7518\u6cb9\u3001\u4e8c\u7532\u82ef\u9752 \u3001\u6eb4\u915a\u84dd\uff09<\/p>\n<p>\uff084\uff09\u67d3 \u6599\uff1a EB\\ Gelred \\ goldview \\ GenGreen \\ GenView \\SYBRGreen\u7b49\u8367\u5149\u67d3\u6599<\/p>\n<p>5 \u8017\u6750\uff1a \u706d\u83cc\u7684\u767d\u67aa\u5934\u3001\u9ec4\u67aa\u5934\u3001\u84dd\u67aa\u5934\u3001200ul\\500ul\\1.5ml\u79bb \u5fc3\u7ba1\u7b49 6 DNA Marker \u6839\u636e\u5f85\u6d4b\u7269\u5206\u5b50\u91cf\u6765\u5b9a<\/p>\n<p>\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u7f13\u51b2\u6db2<\/p>\n<p>\u6709\u51e0\u79cd\u7f13\u51b2\u6db2\u9002\u7528\u4e8e\u5929\u7136\u53cc\u94feDNA\u7684\u7535\u6cf3 Tris-\u4e59\u9178\u76d0\u548cEDTA\u7f13\u51b2\u6db2\uff08TAE) Tris-\u787c\u9178\u76d0\u7f13\u51b2\u6db2(TBE) Tris-\u78f7\u9178\u76d0\u7f13\u51b2\u6db2(TPE)<\/p>\n<p>\u743c\u8102\u7cd6\u51dd\u80f6\u7f13\u51b2\u6db2<\/p>\n<p>\u743c\u8102\u7cd6\u51dd\u80f6\u7f13\u51b2\u6db2<\/p>\n<p>\u4e09\u8005\u4e4b\u4e2d\uff0cTAE\u7684\u7f13\u51b2\u80fd\u529b\u6700\u4f4e\uff0c\u53cc\u94fe\u7ebf\u72b6DNA\u7247\u6bb5\u5728TAE\u4e2d\u6bd4\u5728TBE\u6216TPE\u4e2d\u8fc1\u79fb\u5feb10%,\u5bf9\u4e8e\u9ad8\u5206\u5b50\u91cf\u7684DNA\uff0cTAE\u7684\u5206\u8fa8\u7387\u7565\u9ad8\u4e8eTBE\u6216TPE\uff0c\u5bf9\u4e8e\u4f4e\u5206\u5b50\u8d28\u91cf\u7684DNA\uff0cTAE\u8981\u5dee\u4e9b\u3002\u7528\u4e8e\u5206\u6790\u590d\u6742\u57fa\u56e0\u7ec4\u7684Southern\u5370\u8ff9\u5747\u7528TAE\u7535\u6cf3\u7f13\u51b2\u6db2\u5236\u5907\u51dd\u80f6\u548c\u7535\u6cf3\u3002\u8d85\u87ba\u65cbDNA\u5728TAE\u4e2d\u7684\u7535\u6cf3\u5206\u8fa8\u7387\u8981\u597d\u4e8eTBE\u3002<\/p>\n<p>\u743c\u8102\u7cd6\u51dd\u80f6\u7f13\u51b2\u6db2<\/p>\n<p>TBE\u53ef\u4ee5\u4f7f\u75282-3\u6b21\u5de6\u53f3\uff0c\u800cTAE\u75281-2\u6b21\u5c31\u8981\u66f4\u6362\u3002\u7535\u6cf3\u7f13\u51b2\u6db2\u591a\u6b21\u4f7f\u7528\u540e\uff0c\u79bb\u5b50\u5f3a\u5ea6\u964d\u4f4e\uff0cpH\u503c\u4e0a\u5347\uff0c\u7f13\u51b2\u6027\u80fd\u4e0b\u964d\uff0c\u53ef\u80fd\u4f7fDNA\u6761\u5e26\u6a21\u7cca\u6216\u4e0d\u89c4\u5219DNA\u5e26\u8fc1\u79fb\u3002 \u7535\u6cf3\u7f13\u51b2\u6db2\u521a\u597d\u6ca1\u8fc7\u51dd\u80f61-2mm\u4e3a\u597d\uff0c\u592a\u591a\u5219\u7535\u6d41\u52a0\u5927\uff0c\u51dd\u80f6\u53d1\u70ed\u3002<\/p>\n<p>\u743c\u8102\u7cd6\u51dd\u80f6\u4e0a\u6837\u7f13\u51b2\u6db2<\/p>\n<p>\u4e0a\u6837\u7f13\u51b2\u6db2\u662f\u4e0a\u6837\u65f6\u4e0e\u6837\u54c1\u4e00\u8d77\u52a0\u5165\u6cf3\u9053\u7684\u3002\u4e0a\u6837\u7f13\u51b2\u6db2\u6709\u4e09\u4e2a\u4f5c\u7528\uff1a<\/p>\n<p>1\uff09\u589e\u52a0\u6837\u54c1\u5bc6\u5ea6\uff1b<\/p>\n<p>2\uff09\u4f7f\u6837\u54c1\u5e26\u6709\u989c\u8272\uff0c\u4fbf\u4e8e\u4e0a\u6837\u64cd\u4f5c\uff1b<\/p>\n<p>3\uff09\u5176\u4e2d\u7684\u6307\u793a\u5242\u5728\u7535\u573a\u4e2d\u4ee5\u53ef\u4ee5\u9884\u6d4b\u7684\u6cf3\u52a8\u901f\u7387 \u5411\u9633\u6781\u8fc1\u79fb\u3002 \u4e0a\u6837\u7f13\u51b2\u6db2\u6709\u51e0\u79cd\uff0c\u4f46\u4f7f\u7528\u7684\u6307\u793a\u5242\u57fa\u672c\u90fd\u5305\u62ec\u6eb4\u915a\u84dd\u548c\u4e8c\u7532\u82ef\u6c30FF\u3002<\/p>\n<p>\u743c\u8102\u7cd6\u51dd\u80f6\u4e0a\u6837\u7f13\u51b2\u6db2<\/p>\n<p>\u6eb4\u915a\u84dd\u5728\u743c\u8102\u7cd6\u51dd\u80f6\u4e2d\u8fc1\u79fb\u901f\u7387\u662f\u4e8c\u7532\u82ef\u6c30FF\u76842.2\u500d\uff0c\u8fd9\u4e00\u7279\u6027\u4e0e\u743c\u8102\u7cd6\u51dd\u80f6\u7684\u6d53\u5ea6\u65e0\u5173\uff1b \u57280.5\u00d7TBE\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u4e2d\u6eb4\u915a\u84dd\u7684\u901f\u7387\u7ea6\u4e0e\u957f\u7ea6300bp\u7684\u7ebf\u6027\u53cc\u94feDNA\u76f8\u540c\uff0c\u57280.5%-1.4%\u6d53\u5ea6\u7684\u743c\u8102\u7cd6\u51dd\u80f6\u4e2d\u57fa\u672c\u4e0d\u4f1a\u53d8\u5316\u3002 \u542b\u6709\u7684\u7518\u6cb9\u53ef\u4ee5\u52a0\u5927\u6837\u54c1\u7684\u5bc6\u5ea6\uff0c\u4f7f\u5176\u5927\u4e8eTAE\u7684\u6d53\u5ea6\u800c\u6c89\u964d\u5230\u70b9\u6837\u5b54\u4e2d\uff0c\u9632\u6b62\u6837\u54c1\u98d8\u51fa\u70b9\u6837\u5b54<\/p>\n<p>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/section>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column][vc_column_text]\u5bf9\u4e8e\u5206\u5b50\u91cf\u5927\u7684\u6837\u54c1\uff0c\u5982\u5927\u5206\u5b50\u6838\u9178\u3001\u75c5\u6bd2\u7b49&hellip;<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[25],"tags":[],"class_list":["post-533","post","type-post","status-publish","format-standard","hentry","category-epk","category-25","description-off"],"_links":{"self":[{"href":"http:\/\/ly.com.cn\/index.php?rest_route=\/wp\/v2\/posts\/533","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/ly.com.cn\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/ly.com.cn\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/ly.com.cn\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"http:\/\/ly.com.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=533"}],"version-history":[{"count":2,"href":"http:\/\/ly.com.cn\/index.php?rest_route=\/wp\/v2\/posts\/533\/revisions"}],"predecessor-version":[{"id":536,"href":"http:\/\/ly.com.cn\/index.php?rest_route=\/wp\/v2\/posts\/533\/revisions\/536"}],"wp:attachment":[{"href":"http:\/\/ly.com.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=533"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/ly.com.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=533"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/ly.com.cn\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=533"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}